show Abstracthide AbstractGenomic studies of the ruby-throated hummingbird are warranted and necessary for further understanding of these fine-tuned metabolic systems. Here we produce a chromosome level hybrid genome assembly of the ruby-throated hummingbird. Because many of the proteins involved in cellular metabolism are quite large, we annotated the genome using a combination of Illumina RNA-seq and Oxford Nanopore cDNA sequencing from muscle and liver tissues to identify full coding sequences and multiple encoded isoforms.